Beta Actin antibody                                                                                                                            

Catalog Number:AM-RaActin-100

 Applications 

    WB 1:1000-1:10000, IHC 1:50-1:500, IF/ICC 1:200

    *The optimal dilutions should be determined by the end user.

 Reactivity 

    human, mouse, rat, chicken, goat, sheep, yeast, ochotona curzoniae, camelus bactrianus, carp

 Storage 

    Store at -20°C. Stable for one year after shipment.

    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for-20ºC storage

 Purification 

    Antigen affinity purification

 Immunogen 

    AG14521

 Uniprot    

    P60709

 Description 

    Beta Actin, also named as ACTBand F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1  (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15    k Da cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193).